Sentence examples for transformants were subsequently from inspiring English sources

After complete DNA sequencing of all gel-purified hybrid plasmids, the E. coli strain BL21 DE3) (F- ompT hsdSB rB- mB-)) was transformed with the hybrid plasmids, and ampicillin-resistant transformants were subsequently selected using LB-agar plates supplemented with ampicillin (100 mg/l).

Transformants were subsequently maintained in 1 μM triclosan (LBT).

The embryogenic calli from these transformants were subsequently analyzed with gus histochemical staining.

Candidate primary transformants were subsequently crossed with spore color mutant fus-1 and ascospores were isolated from hybrid perithecia.

Progeny of a transformant are subsequently screened for those with a decreased resistance to the antibiotic, and then for those which have completely lost the marker and the entire vehicle, leaving only the desired sequence modifications between the two genomic border sequences which were targeted.

The S. cerevisiae transformants obtained were subsequently tested to determine if they could grow when galactose was the sole carbon source (Fig.  3).

Preliminary screening of P. pastoris transformants by PCR showed that a few recombinants harboured the pPICZ αB/ α-amylase plasmid from GS115 strain which were subsequently used for protein expression.

All were subsequently dropped.

"Both were subsequently released.

They were subsequently scrapped.

Transformants were observed after 1 2 weeks.