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The pRS314, pRS314DNA2, pRS314dna2K1080E, and pRS314dna2K1080E,E675A transformants were resistant to IR, whereas the transformant containing the pRS314dna2E675A plasmid was sensitive to IR.
Transformants were resistant to ampicillin, cefazolin, and pipericillin/tazobactam but susceptible to third- and fourth-generation cephalosporins.
The transformants were resistant to 2 mg ml-1 G418, with no lag phase, indicative that the rRNA promoter was driving high level expression of the neo gene.
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Most transformants are resistant to drugs for other (unknown) reasons [ 13].
The transconjugants and transformants were highly resistant to amikacin, gentamicin and tobramycin (MICs, ≥256 μg/mL).
Eight transformants were either resistant or intermediate to cefepime, cefotaxime, and all carbapenems tested, but transformant 1100192-TF was susceptible to meropenem (Table 3).
Multicopy clones can be screened by transformants that are resistant to high levels of a selectable marker compound.
The estimated vector copy number in transformants which are resistant to 1 mg mL-1 G418 was approximately 10.
Polymerase chain reaction (PCR) products with the tagged alleles were introduced into the wild-type diploid strain KT1112/KT1113 by transformation; G418-resistant transformants were sporulated and protein extracts from G418-resistant meiotic progeny were tested for the presence of the tagged protein by immunoblot.
The ligation products were transformed into E. coli DH5a-competent cells by heat-pulse transformation, and the antibiotic resistant transformants were selected to sequence AtFatA.
The construct was transformed into tpx mutant by electroporation and gentamicin resistant transformants were selected.
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