Sentence examples for transformants were picked from inspiring English sources

Since yeast cells have the potential to take up several independent library plasmids during the transformation procedure, two independent E. coli transformants were picked per original yeast clone for plasmid isolation.

The transformants were picked up from the triplicate transformations in 7H9 broth (with supplements) containing 50 ug/ml hygromycin.

The transformants were picked up from the triplicate transformations in 7H9 broth (with supplements) containing 50 ug/ml hygromycin for mycobacteria and in LB in case of E. coli.

Transformants were picked from LB plates containing ampicillin at a final concentration of 10 µg/mL as a selectable marker.

Single colonies of both single and double transformants were picked and cultured for two days.

Transformants were picked into 50 ul of ultra-pure water and plasmid DNA liberated by heat lysing.

Three colonies from each transformant were picked for growth curve analysis.

To ensure that each strain was established independently, only one transformant was picked up from each selection plate.

Individual transformant colonies were picked and spotted onto HMM medium and the GFP fluorescence of individual spots quantified using an AlphaImager UV transillumination system (CellBiosciences: [ 15].

Three RQ2/pHX02 transformants (#2, #3, and #4) and three RQ2/pHX04 transformants (#3, #4, and #5) were picked up from SVO plates and grown overnight in liquid SVO medium with 150  μg kanamycin mL−1.

Positive transformants, based on white/blue screening, were picked up and grown at 37°C in 3 ml of liquid LB medium supplemented with ampicillin (50 μg mL-1).