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When Y. lipolytica was transformed with the expression cassettes, purple transformants were observed on the YNB-casamino selective plates compared to the Y. lipolytica negative control transformants (Figure 2).
No transformants were observed on control plates.
Transformants were observed after 1 2 weeks.
Similar variations among independent transformants were observed when the castor FATTY ACID HYDROXYLASE gene (CFAH12) was expressed in the A. thaliana fad2/fae1 double mutant [ 41] or fatty acid elongase (FAE) gene from nasturtium (Tropaeolum majus) was overexpressed in WT A. thaliana [ 42].
Similar(56)
The largest number of transformants was observed with a co-culture time of 2 days, and alterative co-culture times impaired the transformation efficiency (Fig. 2b).
Although OE_g10399 and OE_g1914 showed a decreased percentage (around 10%) in both tests, no significant difference between wild type and transformants was observed.
From 55,000 transformants, 5 were observed to produce a purple color around the colony.
In general, higher transformation frequencies were observed in E. coli O157 H7 9×1022 to 1.25×104 transformants/µg) than in Salmonella (1.2×102 to 1.4×103 transformants/µg).
Transformants expressing GFP were observed under a fluorescence microscope.
Two distinct transformant colony morphologies were observed and correlated with high and null protein production.
In the CAH1 RSP3-HA transformant, swimming CAH1 RSP3-HAbserved as early as 2 hours aftransformant to low CO2.
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