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Although majority of the transformants were not notably different from nontransformants, we did find two transgenic tomato plants that have unexpected flower phenotypes with leaf-like sepals (named Leafy Sepals or LS1 and LS3; Figure 1).
Growth rates and conidiation of the transformants were not changed.
Juvenile transformants were not producing regular fruits five years after flowering for the first time.
These wild-type transformants were not used to calculate the PI.
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The growth rate of transformants was not significantly modified in comparison to the wild type strain indicating that the differences in calculated transformation frequencies were not affected by differences in cell growth or survival (results not shown).
However, the pIKM2 DNA extracted from X514 transformants was not visible on EtBr-stained agarose gels (data not shown), likely due to low plasmid copy numbers in the host cell or inefficient recovery of pIKM2 from the Gram-positive X514 during plasmid DNA extraction.
The mechanism conferring aminoglycoside resistance in the remaining 2 transformants was not caused by armA or rmtC (Table 4) and was not characterized further.
The average rosette area for the four most robust SUC2p::cSUC2 transformants was not significantly different from heterozygous plants (Fig. 2, Table 1) [ 25].
In our study, the occurrence of tetraploid transformants was not specific to any particular construct sequence, as tetraploids occurred among the transformants that yielded regenerants regardless of construct type.
The bioluminescence of heat-shocked transformants was not only strong enough to generate a signal on light-sensitive film, but the luminescence was also visible to the naked eye in the dark room and detectable by a standard digital camera.
Both the fact that processing occurred and the lack of extracellular NucA in the pNuc-cyt control show that extracellular NucA detected in the transformants is not due to cell lysis.
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