Exact(3)
The resulting transformants were monitored by survival kinetics following induction.
The transformants were monitored for colony color phenotype as well as replicated onto plates lacking Ade.
Transformants were selected on RMOP-spectinomycin (500 mg L-1), then subjected to 3-4 rofnds of regeneration on RMOP-spectinomycin/streptomycin (500 mg L-1 ea).. AS5ox transformants were monitored by PCR and DNA gel blot.
Similar(5)
To test the inhibition of yeast-expressed GLUT5, variable concentrations (1 50 µm) of MSNBA were added to YEPF media and the growth of the transformants was monitored.
The mRNA relative abundance of endogenous (CrPSY) and exogenous (CzPSY) phytoene synthase genes of parental and CzPSY-transformant strains of C. reinhardtii was monitored by qRT-PCR, and changes in cellular carotenoids content were determined in order to correlate transcript levels with the biosynthesis of those specific carotenoids.
Transformants were restreaked onto the same medium, and the segregation of the inactivated isiA gene was monitored by PCR, using genomic DNA from the transformants.
Transformants were isolated and the activity of the reporter genes was monitored via growth on SC medium lacking histidine and uracil.
The activity of I- SceI was monitored by formation of sectors without growth after incubating transformants for several days at 30 °C.
Related(1)
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