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The resulting TBPH-GAL4 plasmid was injected into embryos (Best Gene Inc, Chino Hills, CA), and the resulting transformants were mapped and balanced.
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To recover plasmid sequence reads, all GeneHogs transformant reads were mapped using Stampy against reference sequence E. coli DH10B (RefSeq accession no. NC_010473.1).
In a second independent experiment, 2,616 transformant colonies were obtained and 227 fragments were mapped, ranging from 36 to 480 nt (mean = 215 ±13 nt), 132 fragments being common to both experiments.
Several UAS- PACT transformants were obtained and their chromosomal insertions mapped.
Positive transformants were selected and verified by restriction mapping and sequencing.
The plant transformation was performed as described previously59, and the transformants were selected for kanamycin resistance on MS medium.
Positive transformants were selected and confirmed by colony PCR using both insert- and vector-specific primers and restriction mapping.
Transformants were selected on kanamycin-containing LB agar plates.
The transformants were selected on the basis of green fluorescence, total genomic DNA (gDNA) was extracted from these fluorescent transformants.
The transformants were cultured and selected.
Transformants were selected on medium lacking leucine at 32 °C to obtain single colonies.
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