Sentence examples for transformants were initially from inspiring English sources

The PCR fragment was transformed into the strain DS68625-evo and transformants were initially screen on 2 % maltose plate for growth.

Transformants were initially selected on BG-11 agar containing kanamycin at 10 µg/ml, whilst the segregation of clones was performed by restreaking of primary clones on plates supplemented with kanamycin at 50 µg/ml several times (at least three transfers).

Transformants were initially screened for their viability in the absence of histidine.

Transformants were initially selected based on their ability to grow on medium containing phleomycin (100 μg/ml final concentration).

Primary putative transformants were initially screened by PCR for the presence of the hGAD65 gene using GAD specific primers.

Transformants were initially selected on YPD (1% w/v yeast extract, 2% w/v peptone, 2% w/v dextrose) agar plates containing 100 μg/mL Zeocin.

Since, in the absence of pSTB7, haloindole concentrations did not decrease over the course of 30-hour biotransformation reactions, it can be concluded that all haloindole consumed by pSTB7 transformants was initially converted to halotryptophan by the recombinant TrpBA, and that haloindole influx into cells was driven by this conversion.

Recently, an iterative process termed Posttransformational Vector Amplification (PTVA) has been investigated to generate P. pastoris clones containing multiple copies of the entire vector in the genome through the submission of transformants, which were initially selected on a low level of drug and only contained one or a few copies of the vector, to higher levels of zeocin [ 13].

Transformants were selected initially on SC medium minus tryptophan plates, and then on YPG plates, confirming they expressed functional ADP/ATP carriers.

TAR transformants, grown selective medium, were initially screened by colony PCR using primers for the DBL1alpha domain (Table 1).

Doctors were initially mystified.