Sentence examples for transformants were identified from inspiring English sources
The recombinant plasmid was transformed into E. coli JM109, and transformants were identified by restriction digest analysis and sequencing.
3 to 4 transformants were identified for the three transformations that fulfilled these requirements and all of them were assayed for their virulence on tomato leaves.
These effector constructs were directly transformed into the transgenic GUS reporter plants and double transformants were identified through screening for antibiotic (hygromycin) resistance.
For yeast infectivity assays, cell extracts were co-transformed with pRS316 into YJW187 spheroplasts; [ PSI+] URA+ transformants were identified by plating the yeast cells in top agar containing synthetic defined medium lacking uracil and adenine (SD-Ura-Ade) and supplemented with 10 mg/ml adenine hemisulfate (Sunrise Science, San Diego, CA).
Arabidopsis transformation was performed by the flora-dip procedure [ 40] and transformants were identified by screening for kanamycin resistance.
Positive transformants were identified by sequencing (Baseclear, Leiden, The Netherlands).
P[acman]-KO transformants are identified based on the presence of the white+ gene contained within the backbone of the P[acman]-KO 1.0 vector.
Primary switchgrass transformants and co-transformants were identified by PCR with total nucleic acid extracts from leaves of plants in tissue culture (REDExtract Plant PCR Kit, Sigma-Aldrich, St . Louis MO, USA) using primers specific for the coding regions of the transgenes (Table 3) and the amplification conditions described previously [ 7].
Under high stringency screening conditions of 1 × 10 primary transformants, six clones were identified that grew in the absence of histidine and adenine and were β-galactosidase positive.
After testing several thousands of transformants two clones were identified that were unable to grow when the sepF mutant allele was overexpressed by the addition of xylose to the growth medium.
Switchgrass transformation with pMBXS824 yielded 26 bialaphos-resistant callus lines and 78 primary transformants regenerated from them were identified by PCR.
