Sentence examples for transformants were first from inspiring English sources

P. pastoris GS115 was transformed with linearized pHBM306 plasmid DNA and the recombinant transformants were first selected on minimal selective MD medium.

Transformants were first selected for functional β-glucosidase phenotype followed by screening for organic solvent tolerance in acetonitrile, dimethylformamide, dimethyl sulfoxide, methanol, 2-propanol.

The fermentation kinetics of ATCC 824 transformants were first investigated using a pH set-point of 5.0.

For the measurement of bacteria growth rate, transformants were first grown at 37 °C in a shaking incubator with 3 ml liquid LB medium containing 10 g/L tryptone, 10 g/L NaCl and 5 g/L yeast extract.

The transformants were first cultivated at 30°C for 24 hours in a test tube containing 5 mL BHI medium with 25 μg mL−1 kanamycin and then inoculated into 20 mL GP2 medium (Takahashi et al. [2012]) containing 25 μg mL−1 kanamycin in a 200-mL flask for fermentation.

Transformants were first selected by the auxotrophic pDblet (uracil) marker in order to estimate transformation efficiency.

The expression of OsCKX2 in the leaves of selected primary transformants was first analyzed by semi-quantitative RT-PCR (RT-PCR) using specific primers.

Primary transformants (T0) were first planted in the artificial climate incubators (BINDER, Tuttlington, Germany) under standard conditions (28°C day, 20°C night; 12 h light, 12 h dark), and transplanted into the field 5 weeks later.

Transformant colonies were first screened for insertion of the intron within the cpaAIR coding sequence, resulting in an insertion of 915 bp, using two gene-specific primers flanking the predicted 176a intron insertion site.

For each transformation vector, a total of 10 primary T0 transformants from five independent transgenic lines were first screened for the presence of CX3 or CX5 transgene by PCR.

The obtained Y. lipolytica transformants expressing the above CBHI constructs were first characterized using PAGE analysis of their total secretory proteins.