Sentence examples for transformants were digested from inspiring English sources

For Southern blot analysis, genomic DNA (~15 20 μg) of QM9414, the Δ xyr1 strain and the transformants were digested with BamHI or PstI for cel7b, and XhoI or PstI for cel12a copy number determination.

Cloning reaction products were used to transform chemically competent One Shot® TOP 10 E. coli cells (Invitrogen), and miniprep DNA from transformants were digested with restriction enzymes (MBI Fermentas) and analyzed by agarose gel electrophoresis to verify the presence of the gene of interest.

For Southern blot, genomic DNA of the transformants was digested by BamHI and hybridized with the DIG-labeled probes using standard protocols (Sambrook et al. 1989).

Genomic DNA of each transformants was digested by SacI.

To discriminate between independent integration events at different genomic positions and co-integrations at the same locus, genomic DNA of 16 transformants was digested with several restriction enzymes that cut genomic Physcomitrella DNA frequently but do not cut within the nptII cassette, and only rarely within the cDNA sequences carried by the transforming DNA (Fig. 6).

Genomic DNA from each transformant was digested with Nco I, Nde I (both located in the middle of the T-DNA), Eco R I, or both Xba I and Spe I and used as a template for inverse PCR.

Plasmid DNA from each transformant was digested with XmnI (New England Biolabs, Ipswich, MA, USA), separated by electrophoresis, transferred to a nylon membrane (Zeta-Probe; Bio-Rad Laboratories), and hybridized with an 808-bp digoxigenin (DIG labeled blaNDM probe (Table 2) by using the PCR DIG Probe Synthesis Kit (Roche Applied Science, Mannheim, Germany).

3 μg of genomic DNA of IPO323 and transformants obtained with pCeGFP were digested with BglII and separated on a 1.0% agarose gel and capillary transferred to a Hybond N+ membrane (Life Science Technologies, Paisley, UK).

3 μg of genomic DNA of IPO323 and transformants obtained with various vectors were digested with BglII and separated on a 1.0% agarose gel and capillary transferred to a Hybond N+membrane (Amersham Pharmacia Biotech).

Transformant clones were analysed by PCR on colony, and amplified fragments were digested with HaeIII restriction endonuclease.

The PCR amplicons were digested individually.