Sentence examples for transformants were constructed from inspiring English sources
To solve these problems, overexpressions of amy365-1 and vgb in S. bambusicola were investigated and three overexpression transformants were constructed.
Uricase-overproducing transformants of H. polymorpha were constructed using straight-forward microbiological techniques.
Three overexpression vectors were constructed and their transformants were cultured using corn flour as carbon source.
The vectors pET-28a-Aoxyn11A pET-28a-Aoxyn11A pET-28a-Aoxyn11Amands E. coli/Aoxyn11A and P. pastoris/Aoxyn11andere constransformantsr lab (Li E. al. 2013).
In order to increase the use ratio of corn substrate and improve the dissolved oxygen supply, α-amylase gene amy365-1 and Vitreoscilla hemoglobin gene vgb were overexpressed, and three plasmids which included Phygamy365-1, Phygvgb and PhygPgpdAamy365-1-vgb PhygPgpdAamy365-1-vgb PhygPgpdAamy365-1-vgbre obtained.
Three transformants included Phygamy365-1 strain, Phygvgb strain and PhygPgpdAamy365-1-vgb PhygPgpdAamy365-1-vgb PhygPgpdAamy365-1-vgb
A transformant of Moorella thermoacetica was constructed for thermophilic ethanol production from lignocellulosic biomass by deleting two phosphotransacetylase genes, pdul1 and pdul2, and introducing the native aldehyde dehydrogenase gene (aldh) controlled by the promoter from glyceraldehyde-3-phosphate dehydrogenase.
As a control, an A. oryzae transformant secreting triacylglycerol lipase was constructed using the pISI-tglA vector.
A clone library was constructed, and positive transformants (white colonies) were confirmed by PCR with primers M13F and M13R.
Knockout of eqxS FusΔeqx5 was constructed by transforming FusΔeqxC with knockout vector ClusterPhleoKO (Supporting Information) and transformants selected on phleomycin.
Transformants harboring pGK::nucMCS and pGKahpC, which was constructed as described below, were cultivated in the presence of erythromycin (5 μg/mL).
