Sentence examples for transformants were confirmed from inspiring English sources
Twenty-one out of 23 transformants were confirmed containing the vgb gene (data not shown).
The positive transformants were confirmed by colony PCR and DNA sequencing, respectively.
Zeocin positive transformants were confirmed by PCRs with primer sets: 1F and 1R, 2F and 2R, 1F and 2R (Table2R.
The presence of the 870 bp zlaI and 639 bp zlbI genes in the transformants were confirmed by PCR amplification.
The Km-resistant C. glutamicum transformants were confirmed using PCR with the primers CE0634-up300F and CE0635-R (Table 1).
The obtained transformants were confirmed by PCR and subcultured in PDA plates with 100 µg/mL hygromycin B for three generations to test the genetic stability.
High transformation efficiency and accuracy were achieved as ~63% of the transformants was confirmed to be correct.
Expression of tlp in the putative transformants was confirmed by western blotting analysis.
The complete segregation of the transformants was confirmed by PCR.
The presence of 1.5 Kbp P GAP - skc- AOX TT insert in Saccharomyces transformants was confirmed by colony PCR).
The presence of the transgenes in hygromycin-resistant putative transformants was confirmed by PCR using primers g3&g4 and h3&h4 (Table 3).
