Sentence examples for transformants were analysed from inspiring English sources
To assess the efficiency of selection, ssDNA was transformed into E. coli TG1 and 90 individual transformants were analysed by sequencing the phagemid inserts.
Many transformants were analysed in this manner, and were found to have different expression levels of Mad3p (reflecting different copy numbers of integrated constructs).
Primary transformants were analysed for EsWRKY33 expression by RT-qPCR.
Four resultant transformants were analysed for each cloning experiment.
Transformants were analysed by Southern blot for correct integration at the cbh2 locus.
Transformants were analysed on selective media containing 30 or 60 μg/ml tetracycline (Sigma) after incubation at 37°C for 18 24 h.
The transformant supernatants were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and the fusion proteins were clearly observed as bands with the expected molecular weights of 84.8 kDa (including a 48.0-kDa fusion protein tag) for LsrB and 57.4 kDa (including a 48.0-kDa fusion protein tag) for LuxS (Fig. 4).
Transformant clones were analysed by PCR on colony, and amplified fragments were digested with HaeIII restriction endonuclease.
The generated constructs were transformed to Arabidopsis thaliana wild type Col-0 plants, and at least four GFP-expressing stable primary transformants per construct were analysed for their protein localisation.
To investigate the functionality of BoFRIa-1 and BoFRIa-4 alleles under different environmental conditions five transformants carrying each allele were analysed in the next (T2) generation.
Activities of 102 transformants expressing the enzyme Lip2p, a lipase from Y. lipolytica, were analysed.
