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Transformants were also streaked on QDO medium supplemented with 40 µg/ml 5-bromo-4-chloro-3-indoxyl-α-d-galactopyranoside (X-α-Gal) to further confirm interaction of different co-transformants.
All transformants were also tested for growth on glucose.
Furthermore, in root growth assays, the transformants were also hypersensitive to ABA and NDGA as compared with the wild type (Figure 10B, C).
Transformants were also screened for the Δ152 164 mutation by a PCR amplification method that generated either a 209 bp (wild-type) or a 170 bp (Δ152 164 mutant) product (Fig. 2C).
Transformants were also obtained in Diamond's BI-S-33 medium as described below.
Transformants were also re-investigated for the presence of the g-luc reporter gene.
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The long term stability of co-transformed genes in 20 luciferase expressing transformants was also examined.
Successful display of mCherry on the cell surface of cultivated Y. lipolytica transformants was also confirmed by fluorescent microscopy.
Second, the accumulation of ethidium bromide by R6, R6 patB:: magellan2, M184, and the three transformants was also measured.
The nptII positive status of the transformants was also confirmed by nptII dot blot assay (Fig 3B) and by spraying the leaves with paromomycin (Fig 3C).
Moreover, the fluorescence emission of the transformants was also tested after a 3-hour culture in YP-Gal medium (galactose 20 g/L, peptone 20 g/L, yeast extract 10 g/L).
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