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The apparent lack of amplification of any 5.8 kb product from the inverted repeat region of chloroplast DNA with transformants further implies that transformants are all homoplasmic.

All transformants were also tested for growth on glucose.

All the transformants were also cultured on a medium containing 2% glucose at pH 5.8 as a sole source of carbon.

All CmR transformants were also tetR and the new plasmids (pSMP71 and pSMP73) purified using a QIAGEN plasmid prep kit were introduced by transformation into TR7178.

In total, eight transformants were isolated that were all mitotically stable under non-selective conditions.

If the transformant colonies on MacConkey plates were all pale-coloured, the ZFR was deemed to be a recombination-proficient mutant, so the expression plasmid was isolated and the ZFR reading frame was sequenced.

A MET∷trf4 top1Δ strain was viable on restrictive high methionine medium, and multiple top1Δ transformantsrmants isolated in four independent experiments were all growth-impaired but viable.

Paromomycin-resistant transformants were obtained from all the co-bombardments, and, in all transformants, the presence of the aphVIII gene was verified by genomic PCR.

Riboflavin independent transformants were isolated, and all were phenotypically identical to the strain containing the acdX2 allele, confirming that this represents the deletion phenotype.

Five independent transformants were recovered, and all exhibited suppression of QF activity in α/β and γ KCs as assessed with NSyb-QFrco.

Successful double transformants were obtained for all combinations of genes as confirmed using genotyping and RT-PCR, except for the strain TPS-N with addition of CfDXS in neutral site 2, which could not be successfully generated and was therefore left out of the study.

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