Sentence examples for transformants we used from inspiring English sources

To select canavanine-resistant transformants, we used solid omission medium lacking arginine (SD −Arg) containing 120 μg/ml of canavanine.

For selection of transformants, we used media plates containing 1 μg/mL (R6 derivatives) or 8 μg/mL (T1 derivatives) of ciprofloxacin.

As this toxicity could be due to a prion that would appear during the 15 16 generations required to get the transformant, we used an additional strategy.

Therefore, if we used stable transformants of the cre gene for site-directed integration experiments, lox66 should show a lower efficiency because of its higher rate of re-excision.

Plasmid DNA isolated from pools of E. coli transformants were used to transform yeast strain RSy5 as described below.

Total DNA isolated from JWCB018 transformants was used to "back-transform" E. coli and plasmid DNA isolated from these back-transformants was analyzed by restriction digestion.

A sample of 16 randomly-chosen transformants was used to estimate the transgene copy number by real-time qPCR.

Five randomly selected A95 transformants were used for total DNA isolation and amplification of the hph gene.

Supernatants of homogenized mycelium of the transformants were used in PCR amplification with primer pair hptF1-hptR1 (a) or gpdF12-lccR12 (b).

A total of 10 randomly selected transformants were used for PCR verification using the specific primers designed for the hph gene, and the results revealed that an expected band of 500 bp was amplified from all of the 10 transformants.

After 1 to 2 weeks of incubation, spectinomycin resistant transformants were used to inoculate liquid medium.