Sentence examples for transformants we identified from inspiring English sources
As expected, the vast majority of colonies scored as Lac− (white), but, after screening over 10000 transformants, we identified ~36 Lac+ (pink) colonies.
From a screen of roughly 150 transformants by Southern hybridization, we identified a single clone with the correct integration near Tel VR; nearly all other transformants had ectopic insertions, as is typical in Neurospora.
From a pool of 75000 transformants for each block of three Cys, we identified multiple mutants that preserved drug resistance and yeast mating activity.
It therefore appears unlikely that by screening additional transformants we could have identified hACHES lines that would accumulate significantly higher levels of AChE, than those we report.
Using the latter approach, we identified 389 canr colonies from the original 7,200 transformants.
In this paper, we identify two fission yeast fluoride exporter channels (Fex1p and Fex2p) and describe the development of a new strategy using Fex1p as a selection marker for transformants in rich media supplemented with fluoride.
The recombinant plasmid was transformed into E. coli JM109, and transformants were identified by restriction digest analysis and sequencing.
3 to 4 transformants were identified for the three transformations that fulfilled these requirements and all of them were assayed for their virulence on tomato leaves.
These effector constructs were directly transformed into the transgenic GUS reporter plants and double transformants were identified through screening for antibiotic (hygromycin) resistance.
For yeast infectivity assays, cell extracts were co-transformed with pRS316 into YJW187 spheroplasts; [ PSI+] URA+ transformants were identified by plating the yeast cells in top agar containing synthetic defined medium lacking uracil and adenine (SD-Ura-Ade) and supplemented with 10 mg/ml adenine hemisulfate (Sunrise Science, San Diego, CA).
Arabidopsis transformation was performed by the flora-dip procedure [ 40] and transformants were identified by screening for kanamycin resistance.
