Sentence examples for transformants we have from inspiring English sources

Because our initial efforts at transforming Ap have required a substantial amount of in vitro culture (e.g. to generate sufficient quantities of bacteria for transformation experiments and to cultivate potential transformants), we have been concerned that the transformants that arise will be poorly infective for animals.

In a sequence analysis of the Syrian hamster H-ras gene of eight tumor cell lines from radiation transformants, we have not found any mutation in codons 12, 13, 59, 61, nor in the flanking regions of these codons.

It therefore appears unlikely that by screening additional transformants we could have identified hACHES lines that would accumulate significantly higher levels of AChE, than those we report.

As a positive control, we have used transformants with similar construct expressing gene for editing deaminase from lamprey, pmCDA1 (last row).

As a positive control we have used transformants with pESC-LEU plasmid expressing the pmCDA1 gene, encoding for highly mutagenic cytidine deaminase from lamprey [40].

We have generated transformants of C. gloeosporioides expressing Histone1-eGFP fusion protein (H1-GFP) that label nuclei with a strong eGFP fluorescence.

We find that AID and AID* are expressed at substantially lower levels in the yeast transformants than the APOBECs and we have included an extra panel in Figure 1 figure supplement 1 to show this.

From the SPI data set we have used the 600 most depleted cDNAs for this comparison since the two recent large-scale studies based on colony growth of cDNA transformants have identified 454 and 759 inhibitory cDNAs, respectively [8], [12].

Of the 250 His+ clones only 15 of them exhibited growth on 2 mg/ml G418 containing medium, suggesting that these transformants might have acquired 4-7 cofies of kanamycin resistance gene.

Alternatively, the transformants may have acquired more than one YAC.

However, preparation of fungal protoplasts was laborious, and heterokaryotic transformants might have genetic stability concerns [ 10, 11].