Sentence examples for transformants we found from inspiring English sources

In the remaining 3% of the transformants, we found a second integration event had occurred in addition to the targeted sdi1 locus integration (not shown).

In Southern blot of genomic DNA of all transformants, we found a single band at the expected size (5.3 kb; Fig. 1D) confirming integration of the vector pCeGFP into the sdi1 locus in all cases.

Following isolation and transfer of hygromycin-resistant mycelial slices to media containing increasing concentrations of hygromycin B, we found that transformants were able to propagate in the presence of hygromycin B concentrations as high as 300 μg/ml (Figure 3c, bottom panel).

We found that all transformants had deleted URA3 and incorporated all 9 mutations (Fig. 3B, open circles).

We found that ample yeast transformants were typically obtained using ~1 μg each of linearized plasmid and PCR product.

We found that replicating the transformants to fresh YE-G418 plates at least three times and/or streaking to single colonies led to the elimination of cells containing the ORF in most cases.

In a sequence analysis of the Syrian hamster H-ras gene of eight tumor cell lines from radiation transformants, we have not found any mutation in codons 12, 13, 59, 61, nor in the flanking regions of these codons.

Furthermore, induction of genes we found attenuated in sak1 was restored in these transformants, confirming that Cre17.g741300 is the SAK1 gene required for acclimation and the gene expression response to O2.

Furthermore, we found that the Y. lipolytica[CBHII] transformant cells alone cannot utilize Avicel as the sole carbon source.

We found that the increased mutation load in AID* ung1Δ transformants was accompanied by a dramatic shift away from mutational clustering.

We found that the pDEP plasmids could only be reliably transformed into λDE3 cells if the transformants were grown in media (or plated on media) containing a lower amount of amplicilin (25 μg/ml).