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Gene integration into prototrophic transformants was verified by genomic PCR.
The presence of TcAAD gene in the selected transformants was verified by PCR using gene-specific flanking primers.
The presence of TcTrx cDNA in the selected transformants was verified by PCR using gene-specific flanking primers.
The transformants were verified by PCR to determine if integration of the vector construct had occurred, followed by analysis of expression from cDNA (Fig. 2).The quality of the synthesized cDNA of the transformants was verified by amplifying a short terminal fragment of the beta- actin gene as control.
Gene integration into the prototrophic transformants was verified using genomic PCR.
Additionally to the presence of the egfp reporter gene by PCR analysis, expression of the gene in the fungal transformants was verified by fluorescence microscopy analysis.
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The SOK2 gene was modified by insertion of a triple HA-tag sequence and the transformants were verified in a western blot.
The genotypes of BCG transformants were verified by Southern blotting: two of the tested recombinant strains were of the desired genotype carrying a single copy of the plasmid at the attB site in the bacterial chromosome (Fig. S2).
All transformants were verified by PCR.
Transformants were verified by colony PCR and sequencing.
Transformants were verified by PCR and Sanger sequencing.
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