Sentence examples for transformants was used from inspiring English sources

Total DNA isolated from JWCB018 transformants was used to "back-transform" E. coli and plasmid DNA isolated from these back-transformants was analyzed by restriction digestion.

A sample of 16 randomly-chosen transformants was used to estimate the transgene copy number by real-time qPCR.

PCR amplimers were obtained when DNA from M184 or the three transformants was used as a template but not when R6 DNA was used (http://epapers.bham.ac.uk/1958/).ac.uk/1958/

Pulsed-field gel electrophoresis of intact chromosomal DNA prepared from Ura+ transformants was used to confirm formation of full-length CF products (Schwartz and Cantor 1984; Davis and Symington 2004).

However, when DNA from M184 or the three transformants was used as a template and the PCR extension time was increased to 10 min, a second PCR amplimer of approximately 9 kb was observed (http://epapers.bham.ac.uk/1958/).ac.uk/1958/

Plasmid DNA isolated from pools of E. coli transformants were used to transform yeast strain RSy5 as described below.

Five randomly selected A95 transformants were used for total DNA isolation and amplification of the hph gene.

Supernatants of homogenized mycelium of the transformants were used in PCR amplification with primer pair hptF1-hptR1 (a) or gpdF12-lccR12 (b).

A total of 10 randomly selected transformants were used for PCR verification using the specific primers designed for the hph gene, and the results revealed that an expected band of 500 bp was amplified from all of the 10 transformants.

After 1 to 2 weeks of incubation, spectinomycin resistant transformants were used to inoculate liquid medium.

The T2 progeny from these transformants were used in the experiments described in the text.