Six riboflavin independent transformants were tested for co-transformation of pTRcre2 via complementation of these creB1937 phenotypes.
The esa1 -414 temperature-sensitive strain was transformed with the library, and transformants were tested for growth at both permissive and restrictive temperatures.
To verify that the gene encoding siderophore trait is harbored on pSID/EGYII or not, these transformants were tested against E. amylovora strain EGY1 DSM 101800 using well-cut diffusion test.
The transformants were tested for their capacity to grow on uracil or 5FU containing media.
The transformants were tested for growth on M63 minimal medium including 10% (w/v) of glucose.
Notably, at least three independent transformants were tested for the survival plating to rule out clonogenic variation of the effects.
Initially, patches of four independent transformants were tested for growth at different temperatures on SC-ura plates for three days.
Transformants were tested for growth on 5-fluoroorotic acid (5-FOA) plates that select against URA3-based plasmids.
Truncated genes were inserted into the E. coli RL 257 [29] strain, and transformants were tested for altered growth characteristics on a medium containing glucose.
To further exclude a second integration of the LEU2 marker gene in the genome of the generated Δtpi1 strain, the wild-type yeast TPI1 gene was recombined back into the TPI1 locus and transformants were tested for the loss of leucine prototrophy.
