Sentence examples for transformants was sequenced from inspiring English sources

Complementary oligonucleotide primers containing the desired mutation were used to generate POMGnT1 mutant genes (see Supplementary Table S1 at http://www.BiochemJ.org/bj/436/bj4360447add.htm) according to the standard protocol for QuikChange® site-directed mutagenesis (Stratagene); plasmid DNA of transformants was sequenced to verify that selected clones contained the desired mutation.

The transformants were sequenced using an ABI 3700 automated DNA sequencer.

DNA extracted from LNZ-resistant mutants was serially transformed in sensitive isolates and the genomes of these transformants were sequenced.

Subsequently, plasmids isolated from the positive transformants were sequenced and further analyzed with DNAMAN.

Plasmids isolated from these positive transformants were sequenced, and sequence analysis revealed that the TF binding to p01 fragment was catabolite control protein A (CcpA).

Finally, plasmids isolated from the transformants were sequenced to confirm the mutations.

In the pilot experiment, DNA from 90 randomly selected transformants were sequenced at the Massey Genome Service Massey Universityy, New Zealand).

For each gene, 24 transformants were sequenced in forward and reverse orientations.

The QRDRs of several independent transformants were sequenced to confirm the presence of the same mutation in the donor DNA and MICs of these transformants were determined.

The genomes of the 1974M1 mutant and the 1974T3 and R6T2 transformants were sequenced using the massively parallel sequencing 454 Life Sciences GS-FLX systems (Roche).

Transformants were sequenced using primers U1, D1, URA3intF2, and URA3intF3 to identify ones where no mutations were introduced into the URA3 gene during the construction.