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Sixteen independent ligation samples were transformed into E. coli XL2-blue, and a total of 3658 E. coli transformants was selected at 37°C on LB agar containing erythromycin.
Transformants were selected on selective minimal medium as described above.
The resulting verified plasmid was transformed into G27 and transformants were selected on Kan 25.
Transposon-mutagenized plasmids were transformed into DH5α and transformants were selected on LB + Amp + Kan plates.
The transformants were selected on a minimal selective medium.
(2) PCR-fused products were directly used to transform B. subtilis, and Zeor transformants were selected.
DY8430 was transformed with pAW8 and Leu+ transformants were selected.
The transformants were selected by 5-FOA counter selection.
The construct was transformed into tpx mutant by electroporation and gentamicin resistant transformants were selected.
HWH11 strain was transformed by YEp352- ALD4 by lithium acetate methods and URA3 transformants were selected.
Transformants were selected on SD-URA plates.
