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Transformation of B. burgdorferi B313 and characterization of transformants was previously described [30].
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INO expression pattern, as revealed by GUS staining in ovules of P-INO::GUS transformants, has been previously described [ 11, 12].
Procedures for S. gordonii transformation, screening and genetic analysis of transformants were as previously described [ 33].
Mutagenesis and selection of Tn7::UAU1 transformants was performed using primers in Table 4 as previously described [24].
Nevertheless, the genetic stability of the T-DNA cassette in the transformants was comparable to the mitotic stability rate of 40%% previously reported (Mora-Lugo et al. 2014).
The hybridization probe for the southern blot analysis of the TDR transformants was designed to consist of a single fragment containing FADO, TprC terminator, tef1 promoter and FAR sequence, originating from the previously constructed expression vector (Fig. 3a).
One of the Ura+ transformants was named strain TR2-7.
Plasmid DNA from positive transformants was isolated and sequenced.
All yeast transformants (a Yap1p-overexpressing transformant and a control transformant) that were used in this study were previously constructed and stored in our laboratory [ 13].
Potato (cv Desirée) was transformed and transformants were selected on kanamycin and tested via PCR as described previously [36] [29].
pBBE22-dbpA' and pME22-dbpB' were electroporated into ΔdbpAB; resulting transformants were screened as described previously [4].
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