Similarly, Ade+ Ura+ or Leu+ Ura+ transformants were only rarely recovered from transformation with the replicating plasmid pRS416.
The CzPSY transcript level reached 140-fold the level of the endogenous PSY of the wild type in the transformants T1 and T10, however the violaxanthin and lutein contents in these transformants were only 2.0- and 2.2-fold higher than the parental strain, respectively, in T10 transformant, and 7% and 21% higher, respectively, in T1.
Accordingly, the glucose consumption rate of the MstG transformant in the 50 mM condition was much faster, being able to deplete the monosaccharide after around 40 h of growth, whereas in the same time-span the MstH transformant was only able to consume a 60%% of the total amount suggesting that MstG and MstH are glucose transporters with different affinities for the sugar.
From the above, it is estimated that co-transformant L 61 was only able to oxidize phenolic residues that were presented at surface of lignin molecules in Japanese cedar wood.
The bioluminescence of heat-shocked transformants was not only strong enough to generate a signal on light-sensitive film, but the luminescence was also visible to the naked eye in the dark room and detectable by a standard digital camera.
One might speculate on the repressing activity of G. graminis LOX on ppoC, since PpoC was only slightly present in the A. nidulans transformant.
Their first transformants were isolated only recently after decades of efforts [ 3].
Although the T2245C substitution was present in these three resistant strains, clarithromycin-resistant transformants containing the T2245C substitution were only observed after transformation with genomic DNA, but not after transformation with 23S rRNA PCR fragments.
Finally, a library size of 2.6 × 10 transformants was obtained, while the control reaction with only linearized plasmid gave 4 × 10 transformants.
Furthermore, transformation protocol for generating a large number of transformants is limited to only a few cultivars, indicating that experimental parameters for rice transformation have not been fully optimized (Ozawa 2009).
