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A VHH library of about 10 independent transformants was obtained.

Genomic DNA from yeast transformants was obtained as described by Querol et al. (1992).

Transcript abundance of CmPT1 in the confirmed primary transformants was obtained by qRT-PCR, as described above.

Finally, a library size of 2.6 × 10 transformants was obtained, while the control reaction with only linearized plasmid gave 4 × 10 transformants.

A library containing 6 × 10 transformants was obtained and screened against CAT-8015 using the same strategy as that explained above.

Fifteen and 23 transformants were obtained from transformation with the constructs.

More than 100 positive transformants were obtained after transforming the silencing construct into C. reinhardtii CC425.

Using this technique, up to 145 transformants were obtained per transformation plate, and a ku70 deletion strain was constructed in the ATCC 42464 background and characterized.

During development of the transformation protocol, transformation parameters were modified until paromomycin-resistant transformants were obtained (see below).

The six different modified 35S constructs were introduced through Agrobacterium-mediated transformation and 194 lines of independent transformants were obtained.

Even, no transformants were obtained when plasmid p602/22lux was transformed into B. subtilis BR151.