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Under fluorescent microscopy, the visibility of the pLyc027 transformants was much more obvious in the cellulose medium than in the cellobiose medium.

For example, at maturity, the transformants were much smaller than the wild type and their leaf trichomes were reduced in size and branching (Figure 10A).

For the transformants, expressing the cphA6803-encoding gene was much higher, ranging from 0.8%to39.5%5% of the PDC transcript.

Strikingly, in all cases, the number of transformants with loss-of-function phenotypes was much larger than those with an overexpression phenotype.

Accordingly, the glucose consumption rate of the MstG transformant in the 50 mM condition was much faster, being able to deplete the monosaccharide after around 40 h of growth, whereas in the same time-span the MstH transformant was only able to consume a 60%% of the total amount suggesting that MstG and MstH are glucose transporters with different affinities for the sugar.

Both transformants had a specific enzyme activity of 1.5 mU/mg proteins, which was much higher than the wild-type in which an activity of 0.27 mU/mg protein was measured (Table  4).

As already described in the results, the final ethanol production for the CN6 strain was much lower in comparison to the rest of the transformants tested (Figure 2).

One of the Ura+ transformants was named strain TR2-7.

Plasmid DNA from positive transformants was isolated and sequenced.

Genomic PCR on independent transformants was performed, and 15 independent lines of T0 tobacco transformants were obtained.

In general, the OsCKX2 transcript levels were much lower in the transformants than in WT (Fig. 1b), except for line 6 1 of the CX3-suppression line (Fig. 1a).