Sentence examples for transformants was initially from inspiring English sources

Since, in the absence of pSTB7, haloindole concentrations did not decrease over the course of 30-hour biotransformation reactions, it can be concluded that all haloindole consumed by pSTB7 transformants was initially converted to halotryptophan by the recombinant TrpBA, and that haloindole influx into cells was driven by this conversion.

The PCR fragment was transformed into the strain DS68625-evo and transformants were initially screen on 2 % maltose plate for growth.

Transformants were initially selected on BG-11 agar containing kanamycin at 10 µg/ml, whilst the segregation of clones was performed by restreaking of primary clones on plates supplemented with kanamycin at 50 µg/ml several times (at least three transfers).

Transformants were initially screened for their viability in the absence of histidine.

Transformants were initially selected based on their ability to grow on medium containing phleomycin (100 μg/ml final concentration).

Primary putative transformants were initially screened by PCR for the presence of the hGAD65 gene using GAD specific primers.

Transformants were initially selected on YPD (1% w/v yeast extract, 2% w/v peptone, 2% w/v dextrose) agar plates containing 100 μg/mL Zeocin.

Transformants were initially selected on Trp- medium and then screened or selected on FOA medium as described above for SSG mutagenesis.

Transformants were initially selected by MD medium (1.34% yeast nitrogen base, 4 × 10−5% biotin, 2% dextrose) plates and then checked by colony PCR.

Transformants were initially selected in the presence of 1 μg/ml geneticin (Invitrogen), and the geneticin concentrations were gradually increased to 7 μg/mL during the subsequent two weeks prior to subjecting the transformants to analyses.

Each transformant line was initially expected to contain a trigger gene as follows: A6 (PcNLP2), A13 (PcNLP10), O18 (PcNLP15), M1 (PcNLP6), H6 (PcNLP9), S5 (PcNLP14), S27 (PcNLP13).