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The presence of CX3 (or CX5), hptII and GUS from selected primary transformants was detected by PCR.
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However, for some reason the frequency of transformation of the wild-type strain HS143 to StrR was 4 log orders lower compared to the frequency of transformation to KanR or ChlR, and no transformants were detected with either the ApΔcomE1 or the complemented mutant using the StrR donor DNA.
As given in Table 6, no transformants were detected with any combinations of strains tested.
Colony forming units (CFUs) of the transformants were detected in the presence of inducer.
In contrast, the strongest western blot bands in the homogenate of the D591V transformant were detected in fractions 11.5 and 12 mL, where proteins with molecular masses of 170 kDa were predominant (Fig. 2B).
With the exception of the 133 bp transformants, GUS activity was detected in flowers and minimal activity was present in the leaves.
In addition to the pPmr3-transformants, the aphVIII gene was detected on Southern blots with genomic DNA from transformants that were produced with plasmid paphG (data not shown).
Phenol-oxidizing activity was detected from transformants of the yeasts, indicating that the obtained cDNA encodes a laccase.
Previously, no PFK-M activity in these transformants grown on complex medium was detected.
No pullulanase activity was detected in uninduced transformant or from transformant harboring the empty pET plasmid.
In contrast, luciferase activity was detected in several transformants generated by co-transformation with the plasmid pHRLucP.
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