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Immunoprecipitation experiments revealed that Rubisco activity detected in the transformants was derived solely from RbcTk.

All clones contained the same 9-bp duplication (ATTTTATGG), indicating that the Rmontanensis700 transformant population was derived from a single transposon integration event.

The following new transgenic lines bearing mutant enhancers were generated: UbxBR was derived from mutating UbxB (in a ry + vector; Thuringer et al., 1993), and 2 independent transformant lines were isolated by standard procedures.

Transformants of Fusarium graminearum were derived using linearized DNA of plasmids designed to replace the trichodiene synthase gene, a cutinase gene or a xylanase gene with a hygromycin-resistance marker cassette by homologous recombination between 1-kbp segments of flanking DNA.

The variation of transformant cell density may be derived from different loci and copy numbers of the integrated transgenic fragments [ 5, 26, 27].

However, these transgenic lines were derived from the same transformant vector, which were unable to distinguish them from each other using common methods.

Glycerol stocks (8%) of transformants were stored at −80°C, and experimental cultures were derived from single colonies grown overnight on LB agar at 37°C.

Spotting assays were derived from a pool of three independent fresh transformants.

Plasmid DNA was readily isolated from C. hydrothermalis transformants (perhaps indicating a high copy number in C. hydrothermalis as it is derived from a high copy number C. bescii native plasmid [ 13, 37]) and used to transform C. bescii.

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