Sentence examples for transformants was carried from inspiring English sources

Moreover, PCR amplification for these 10 transformants was carried out using specific primers designed for the Vir gene of A. tumefaciens, with four strains of A. tumefaciens as positive controls for eliminating the false-positive caused by the contamination of A. tumefaciens.

A Western blot analysis of KmR transformants was carried out using the anti-SPA monoclonal anti-FLAG M2 antibody (Sigma, St Louis, MO).

Selection of the primary transformants was carried out on MS medium containing 200 μg/ml kanamycin.

Transformation of naturally competent cells of S. gordonii V288 and GP201, scoring and genetic analysis of transformants was carried out as already described [ 21, 22].

Electroporation and selection of transformants were carried out by using MD and G418.

First, two steps screening of the transformants were carried out.

Fed-batch fermentation of E. coli Trans 1-T1 transformant was carried out in a 2000 L fermentor to product recombinant ESH.

Bioreactor production of the best-producing transformant was carried out in a 2-l bioreactor Tryton (Pierre Guerin, Mauze, France) according to the P. pastoris fermentation process guidelines (Invitrogen).

Transformation efficiency was carried out by LiCl- polyethylene glycol, and transformants grown on SD/-Leu, SD/-Trp were tested for β-galactosidase activity.

The cultivation of the two transformants Y and C was carried out with a multi-bioreactor system (Sixfors from Infors AG, Bottmingen, Switzerland).

Selection of His+ transformants and G418-resistant clones was carried out according to the manufacturer's instruction manual (Invitrogen, USA).