Sentence examples for transformants was assayed from inspiring English sources

The level of transcription of Chi67-1 in the transformants was assayed using an IQ5 Multicolor Real-Time PCR Detection System (Bio-Rad, CA, USA) and SYBR Premix Ex Taq (Takara, Dalian, China), with the elongation factor EF1 as a reference gene (Sun et al. 2015a).

The C3b cleavage capacity of B. garinii transformants was assayed after incubation of spirochetes (4×107) with PBS supplemented with 750 ng/ml purified CFH for 60 min at room temperature (Calbiochem, Darmstadt, Germany) as described previously [43].

The cbh1 expression cassette in poswo1 transformants was assayed by PCR using the primers CBHI-F: 5'-CAGCGTACCCGTACAAGTCGTAATC-3' and CBHI-R: 5'- TGGTACTGGGATACACGAAGAGCG-3'.

Cultures of the largest colonies (1% of the transformants) were assayed for activity towards paraoxon spectrophotometrically in microtitre plates.

Three transformants were assayed in duplicate per strain.

All transformants were assayed for inducible expression of luciferase.

Following cell lysis, the transformants were assayed for luciferase activity in 24-well tissue culture plates.

Levels of Ppα-DOX transcript accumulation of the selected transformants were assayed by Northern blot analysis.

The transformants were assayed for their ability to activate transcription from the GAL4 upstream activation sequence.

Cellulase activity assays Cel7b transformants were assayed using 4-methylumbelliferyl-β d-cellobioside (MUC) and cel7a transformants using 4-methylumbelliferyl-β d-lactoside (MULAC) as substrate.

The PCR-amplified coding sequence is inserted by homologous recombination into a yeast expression vector system, and transformants are assayed for growing in a nutrition-deficient medium.