Sentence examples for transformants using a from inspiring English sources
To obtain quantitative expression data of the co-transformed luc gene, we analyzed the bioluminescence of pPsaD-GLuc-derived transformants using a luminometer; in pPsaD-GLuc, the luc gene is under control of the psaD promoter from C. reinhardtii.
Plasmid DNA was extracted from Thermoanaerobacter sp. X514 transformants using a Qiagen plasmid mini-preparation kit.
For comparison, we also generated pPsaD-GLuc-derived transformants using a different parent G. pectorale wild type strain, CCAP 32/14.
Excision of L5 was confirmed in pREP41-Cre transformants using a PCR strategy (BWP33F, BWP33R, and BWP246F), followed by Southern blot analysis.
We investigated the detection of light in heat-induced transformants using a digital camera and a light-sensitive film in order to optimize the assay for high-throughput setups.
Fosmid DNA was extracted from Escherichia coli transformants using a Montage BAC96 MiniPrep kit (Millipore, Billerica, MA, USA), and end sequencing was carried out using dye terminator chemistry on an ABI Prism 3730 as described previously.
Moreover, growth defects of transformants using an inducible promoter for laccase expression were observed only when transformants were cultured under induction conditions.
Compared to the transformant TYEGLAC3-1 expressing the lac3 gene by the constitutive GPD promoter, transformants using an inducible GAL1 promoter revealed higher levels of laccase activity.
To select transformants using an amino acid auxotrophic marker, the appropriate complete minimal dropout medium was used, which contains 43.7 g/l drop out base with agar (DOBA), double the recommended amount of the appropriate complete supplement mixture (CSM) dropout mixture (MP Biomedicals, Santa Ana, CA, USA) and 100 mg/l adenine hemisulfate.
Each well was inoculated with a single His+ transformant using a sterile toothpick and stirred to resuspend the cells.
The same BMMY medium for the above baffled culture-flask was used in the cultivation of P. pastoris transformant using a disposable culture-bag made of polypropylene.
