It was necessary to include the Δ rid mutation to avoid mutating the tcu-1 or nit-6 promoters during crosses of the transformants to wild-type.
The smaller silique size and lower seed number per silique of the AtHMA4 FL-transformants compared to wild-type is likely to be a consequence of the reduced Zn levels in the shoot and hence lower levels reaching the silique.
Thus, the median overall IMD actually 'increases' from 13 kb in AID* wild type transformants to 41 kb in the ung1Δ cells despite the increase in mutation load.
By overexpression of the genes encoding these enzymes, NADPH enhancement was confirmed in the transformant cells as compared to wild type cells, as was the acceleration of lipid accumulation.
Therefore, transformants were crossed to wild-type strain 74-OR23-IVA 74-OR23-IVA 74-OR23-IVA ascospore progeny.
Therefore, transformants were crossed to wild-type to purify homokaryotic meiotic progeny for the knockout project (Colot et al. 2006; Park et al. 2011a).
Addition of bicarbonate to the P. tricornutum culture increased biomass of the transformants by about 12% compared to wild type, and their maximum specific growth rate in exponential phase was about 10% greater than that of wild type.
In individual experiments, the mean level of adhesion and standard deviation from triplicate wells of each transformant were compared to wild-type cells by ANOVA with the Dunnett post test.
We saw large increases in saccharification yields in all transformants compared to the wild type of up to 49% and a wild type like growth phenotype for the lines complemented with the pVND7 IRX7 construct.
Though we have undertaken great effort to replicate the in planta experiments reported here using wild-type I-CreI, we have been unable to obtain Arabidopsis transformants with the wild-type endonuclease gene, perhaps due to leaky expression of the endonuclease resulting in toxicity.
The pattern exhibited by the four wild-type transformants was wild type, except for an additional band corresponding to the pMoCosX vector.
