Sentence examples for transformants to identify from inspiring English sources

For example, in standard SSG mutagenesis, it is necessary to replica plate hundreds of transformants to identify a sufficient number of FOAR isolates that have replaced the marker with the PCR fragment.

Using a replica based screening, twenty thousand yeast transformants were screened to identify transformants expressing heterologous gene sequences from J. curcas with enhanced ability to tolerate stress.

A toothpicking analysis on 100 200 transformants allowed us to identify Ts EmR PmS cells.

In this study, the whole-genome sequences of M184 and three second-generation transformants were analyzed to identify mutations that might be responsible for patAB overexpression.

To determine whether the enhancers within the T-DNA sequence are able to cause an increase in transcript accumulation of endogenous genes, these transformants were examined to identify T-DNA insertion sites within ~5.5 Kb of a gene.

The corresponding cys-loop LGIC PCR products were cloned into the pGEM-T Easy vector (Promega) and between 10 to 20 transformants were sequenced to identify individual subunit isoforms.

Plasmid DNA was sequenced to identify transformants of each allele.

T3 plants were tested again to identify transformants homozygous for both HygR and PPTR.

We used two pairs of primers to identify transformants containing a human HPRT sequence.

The primers were used to identify transformants that were homozygous for the T-DNA insertions yielding the irx9-2, irx7 and irx8 mutants.

These colonies were then replica-plated onto both selective medium and minimum medium plates with 5-fluoroorotic acid (5-FOA, 1 g/l) to identify transformants with stably integrated ura4+, which are inviable in 5-FOA medium [ 27, 28].