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In caraway, the cotyledonary-node transformation method yielded confirmed transformants through a seemingly direct shoot regeneration process.
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In the yeast Saccharomyces cerevisiae, Resnick et al. measured the repair of a linearized leu2 marker locus in yeast chromosomal DNA leading to Leu+ transformants through recombination with RNA oligonucleotides complementary to the broken ends that were transformed into the cells [45].
Clones were isolated from yeast transformants through separation of the YAC DNA from endogenous chromosomes by CHEF (contour clamped homogeneous electric field) gel electrophoresis [48].
The positive transformants were assessed through a seral dilution on the SD/-Leu/-His medium supplemented with different concentrations of 3-amino-1,2,4-triazole 3-AT).
As control, a sample of transformants with an altered morphology was purified through single conidial isolation.
As a result, transformants usually display a mitotically unstable phenotype.
Cells that also acquired a particular cDNA segment could be detected among the G418-resistant transformants by a second selection or by a variety of screening protocols.
All the uidA transformants had the "yellow-in-the-dark" phenotype characteristic of chlL mutants, whereas homoplasmic nifH transformants exhibited a partial "green-in-the-dark" phenotype.
All transformants harboured a single copy integrated into the rDNA by a homologous recombination.
We characterized Arabidopsis and chrysanthemum transformants bearing a CmActin promoter-β-glucuronidase (GUS) reporter construct.
Among the obtained 120 constructs expressing cDNAs, 34 transformants showed a stable phenotype with quicker growth.
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