Sentence examples for transformants the expression from inspiring English sources
In transformants, the expression increased by a factor of 7 to 21 after the switch to nitrate.
According to the results of SDS-PAGE and Western blot analyses, compared with E. coli BL21 DE3) transformants containing pET-29a, E. coli BL21 DE3) transformants containing the expression vector pET-AGAB-4 expressed the rAgaB-4 with the His-tag (attached to the C-terminal end); the molecular weight of the band was similar to the expected molecular weight of 97.32 kDa (Fig. 1).
Y. lipolytica transformants harbouring the expression cassettes showed a purple colour phenotype on selective YNB-casamino plates as compared to control cells indicating that mCherry was displayed on the cells.
Transformants with the expression cassettes for EGFP- or dsRed-fused α-Syn or Synphilin-1 were inoculated in tubes on SCD to OD600 0.1 and incubated for 16 hours at 30°C as described previously [41].
Stable transformants showed the expression of GFP as detected by confocal microscopy.
Interaction of the two fusion proteins in co-transformants drives the expression of the yeast His3 gene, which is required for growth of the histidine-auxotrophic E. coli host on selective plates (see Materials and methods for details).
When Y. lipolytica was transformed with the expression cassettes, purple transformants were observed on the YNB-casamino selective plates compared to the Y. lipolytica negative control transformants (Figure 2).
Importantly, we found that glucoamylase production levels were much more consistent among the transformants that harboured the expression cassette at the targeted locus as compared to transformants with a random integration pattern.
Subsequent to the choice of suitable expression vectors and proper host strains, and transformation of the expression cassettes, it is important to select for transformants which show high expression levels of the desired protein.
This may be due to a random insertion of the recombinant plasmid that may have affected the sporulation pathway of the transformants and modified the expression of sporulation-related genes (Park et al. 2013; Yu et al. 2015).
The I-SceI enzyme mediated integration, or SEMI, in combination with a Δtku70 mutant has a synergistic effect on homologous recombination efficiencies as 90 100% of the transformants exhibited integration of the expression cassette at the homologous site.
