Your English writing platform
Discover LudwigSuggestions(1)
Exact(1)
The first approach consists in making a series of random mutations in the gene that codes for the target protein, creating a library of transformants that will then be screened for a specific property [ 70, 73].
Similar(59)
Because our initial efforts at transforming Ap have required a substantial amount of in vitro culture (e.g. to generate sufficient quantities of bacteria for transformation experiments and to cultivate potential transformants), we have been concerned that the transformants that arise will be poorly infective for animals.
So a good method for the screening and selection of high-producing transformants is certainly very useful that will ultimately decrease the cost of the industrial production.
Transformants that were generated through co-transformation with plasmid pHsp70A-GLuc were also analyzed qualitatively for luciferase activity, and several of these transformants showed chemiluminescence (data not shown).
Transformants that were generated through co-transformation with plasmid pPsaD-GLuc were analyzed for luciferase activity.
Transformation of DGLA-producing A. thaliana with this construct yielded transformants that successfully produced prostaglandin F.
It will be interesting to examine whether any putative nucleases have mutated in the few viable transformants that carry both spacer #1 and its target.
The primary transformants that were fertile (four T0 lines) carried an in frame deletion (multiples of 3) and wild type allele for SlTRM3/4 and an in frame or frameshift mutations for SlTRM5.
The donor fragments each encode a URA3 gene as a positively selectable marker; yeast transformants that are His+ Ura+ are guaranteed to have picked up at least one donor fragment during the in vitro recombination reaction.
The transformants that were able to grow on resistant plates were considered genetically stable.
Leu+ transformants were replica-plated onto YPD plates at 36°C, and the plasmid DNA was recovered from transformants that exhibited plasmid-dependent rescue.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com