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Likewise, we were not able to detect the HA-tagged Hsp70A protein in transformants that were co-transformed with plasmid pHsp-HA, since there was no detectable signal in western blots using anti-HA antibodies (data not shown).
Kolmogorov-Smirnov normality test (Lilliefor's test) was first applied to the quantification data gathered from the 24 independent transformants that were regenerated per transformed construct (96 in total).
Transformants that were generated through co-transformation with plasmid pPsaD-GLuc were analyzed for luciferase activity.
Transformants that were generated through co-transformation with the ars plasmid ptubar4 were analyzed for arylsulfatase activity.
Transformants that were generated through co-transformation with plasmid pHsp70A-GLuc were also analyzed qualitatively for luciferase activity, and several of these transformants showed chemiluminescence (data not shown).
The transformants that were able to grow on resistant plates were considered genetically stable.
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This is in contrast to transformants that are obtained by targeted transformation approaches or mutants obtained by insertion mutagenesis.
Multicopy clones can be screened by transformants that are resistant to high levels of a selectable marker compound.
The screening conditions select yeast transformants that are capable of survival under stress (acquired due to expression of J. curcas heterologous cDNA) and are able to grow; while eliminating events due to lethality, non-expression and other experimental artefacts.
Visual inspection of the filtered alignment identified a region with a high density of improperly paired reads upstream of patA in M184 and the transformants that was not present in R6 (Fig. 2a).
Proteomics data revealed production of three proteins in the A. nidulans transformant that were not found in the wild type.
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