Sentence examples for transformants that may from inspiring English sources

To avoid false positive transformants that may be generated from direct screening for glyphosate tolerance, T-DNA mediated transformants were first screened for kanamycin resistance, a marker carried from the vector pBI121.

Additionally, all of these methods require time-consuming tissue culture steps that are not compatible with high-throughput generation of mutants, and still can produce chimeric transformants that may not pass the trait on to the next generation.

It was found that up to 90% of clones in yeast homologous recombination libraries may be multiple vector transformants, that on average these clones bear four or more unique mutant genes, and that these multiple vector cells persist as a significant proportion of library populations for greater than 24 hours during liquid outgrowth.

Transformants that were generated through co-transformation with plasmid pHsp70A-GLuc were also analyzed qualitatively for luciferase activity, and several of these transformants showed chemiluminescence (data not shown).

Transformants that were generated through co-transformation with plasmid pPsaD-GLuc were analyzed for luciferase activity.

This may be due to a random insertion of the recombinant plasmid that may have affected the sporulation pathway of the transformants and modified the expression of sporulation-related genes (Park et al. 2013; Yu et al. 2015).

Particle bombardment can also be used to obtain transformants with variable success rates [ 8, 9], but can also introduce multiple copies that may recombine or result in co-suppression [ 10].

Transformation of DGLA-producing A. thaliana with this construct yielded transformants that successfully produced prostaglandin F.

Oosumi et al. [ 4] did not report the occurrence of tetraploidy among their transformants; however, a careful examination of the four transformants that were photographically documented by these authors suggests to us that the transformant depicted in their Figure eight-b [ 4] may be a tetraploid, as suggested by the distinctive leaf morphology that we have shown to be indicative of tetraploidy.

The transformants that were able to grow on resistant plates were considered genetically stable.

Leu+ transformants were replica-plated onto YPD plates at 36°C, and the plasmid DNA was recovered from transformants that exhibited plasmid-dependent rescue.