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The 14-mer left and 15-mer right probes were used to select plasmids containing the correct insert, and transformants that did not hybridize with both the left and right probes were omitted.
Two 14-mer left and right probes were used to select phagemids containing the correct insert, and transformants that did not hybridize with both the left and right probes were omitted.
The 14-mer left and 15-mer right probes were used to select phagemids containing the correct insert, and transformants that did not hybridize with both the left and right probes were omitted.
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Sequencing of the Ses locus in these two transformants that do not display Secteurs confirmed the presence of both alleles, i.e., the wild-type allele at the resident locus and the mutated allele s1 at an ectopic position.
The plasmid recovered from the last transformant that did not fluoresce had a truncated insert.
From 2 × 10 primary transformants, 18 were encoding true positives that did not activate transcription in the presence of a non-specific test bait.
Moreover, the proteins carrying these single mutations enabled growth of E. coli transformants encoding mutated human PFK-M in a glucose-containing medium that did not support the growth of E. coli transformed with native human PFK-M.
From 1 × 10 primary transformants and 27 putative positive clones, 11 were encoding putative true positives that did not activate transcription in the presence of a non-specific test bait.
Co-transformants carrying pDsRED-SKL and pCAS2-GFP displayed a punctuated red fluorescence pattern of the peroxisomes that did not overlap with the green fluorescent tubular structures of mitochondria (Figure 4B).
To differentiate between promoters dependent on the regulator and other promoters, plasmid DNA from a pool of the kanamycin-resistant transformants is transformed into bacteria that do not express the transcriptional regulator.
Several transformants were isolated that did not acidify the medium, and these mutants were shown to be deleted in the laeA gene by Southern blot.
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CEO of Professional Science Editing for Scientists @ prosciediting.com