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Qualitative assessment of four independent transformants shows that the 'vector only' has no discernible effect on mutation rates of lys2∷InsE-A 14.
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The karyotype analysis of transformants showed that chromosomes XIV, III, and IV, or other chromosomes are split.
Analysis of culture supernatants from methanol-induced yeast transformants showed that the protein fusion was effectively processed.
Southern blot analysis of transformants showed that the hs2 transgene copy numbers ranged from 1 to 5. Northern blot hybridization confirmed transcription of the transgenes, and western blot determined the HS2 protein expression.
Our control experiments (Figure 1) using defined mixtures of cDNA transformants show that SPI can yield data sets in which real positives overwhelmingly dominate the readout.
Inoculation with P. infestans isolate 90128 on in vitro transplants of primary transformants showed that Rpi-sto1 transformant plantlets remained resistant, whereas Desiree control plantlets started sporulating after 7 days, and turned completely diseased at 14 20 days (Figure 3).
Agarose gel analysis of transformants showed that they carried only a single, large plasmid.
Dot-hybridization of transformants showed that approximately 3% of the clones were alphoid DNA-positive.
However, comparison to pyrG + complemented transformants showed that the increase was the results of complementation of the uridine deficiency alone.
Northern blot analysis of cultivar (cv) 'Purple' transformants showed that eight lines were transgenic for the hygromycin selectable marker.
The molecular analysis of transformants showed that the integration was ectopic (data not shown), supporting the hypothesis that SesB alone is per se a functional gene involved in Secteur development.
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