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Positive transformants show a brown color around the mycelium.

Both rbcL-luxCt/rbcL wt and rbcL-luxCt/rbcL- transformants show a luxCt hybridizing band at the expected size of 4.4-kb, while the WT strain shows no signal with the luxCt probe.

Although transformants show a wild type phenotype under the light microscope, a glow is visible even to the naked eye in the darkroom when the coelenterazine substrate is added to cell lysates of NO3−-induced transformants.

In the light microscope, all transformants show a wild type phenotype without any detectable morphological differences in comparison to the parent strain SAG 12.85, but when the coelenterazine substrate is added to the lysates of these transformants, a glow is visible to the naked eye in the darkroom.

Among the obtained 120 constructs expressing cDNAs, 34 transformants showed a stable phenotype with quicker growth.

Fruits of the transformants showed a significant increase of β-carotene, β-cryptoxanthin and zeaxanthin.

Genotypes of all transformants showing a phenotype different from the control strain were further confirmed by Southern analysis.

Positive transformants showed a band with 2898 bp length, in contrast to the wild type fragment of 1679 bp (data not shown).

Three native gene transformants showed a slightly lower average PDH activity of 0.28 U mL−1 but clone n-PDH_natCC11 had a volumetric activity of 0.47 U mL−1.

The cab12 promoter of 4 transformants showed a specific activity from 223 to 11656 pmol Mu × min-1 × mg-1, respectively (Fig. 4B).

The cab11 promoter of 12 independent transformants showed a specific β-glucuronidase (GUS) activity from 9 to 40599 pmol Mu × min-1 × mg-1, respectively (Fig. 4A).