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To reduce the chances of having single copy transformants, regeneration time should be kept short and transformants should be plated directly on increased concentrations of antibiotic.

This can be done by rescuing the plasmids from yeast transformants into E. coli, isolating the plasmids, and retransforming the original host strain (all transformants should now be compound resistant).

High-efficiency competent cells should be used when transforming; a higher sample of transformants should thus be obtained and current recombinant clone proportions could be confirmed.

It should be noted that all transformants exhibited the same morphological phenotype as their wild-type parental strain.

The automated quantitative detection of GFP-tagged transformants or transconjugants in natural biofilms or aggregates should be explored.

Future studies should be focused on improving the stability of the transformants.

It should be noted that the luciferase activity levels in co-transformants were quite variable.

Fluorescence should not be detected in the transformants where the target gene (ku70) was properly replaced by the bar cassette (Fig.  4a).

Frozen stocks of single transformants and pools of transformants were prepared by standard methods.

Further studies should therefore be carried out to determine the safety of this C. rosea transformant and further assess its capacity to control soybean Sclerotinia stem rot.

These transformants were named transformant cphA6803# 1and transformant cphA6803 2.