Sentence examples for transformants selected for from inspiring English sources
Mutagenic PCR products were transformed into Y270 as previously described [ 65], and G418-resistant transformants selected for on YPAD containing 200 μg/ml G418 (GIBCO).
The three synthetic gene transformants selected for the rescreen yielded an average volumetric PDH activity of around 0.31 U mL−1.
BIR efficiency was determined using 500 ng of the specified linearized CVF or 100 ng of circular replicative plasmid pRS416 for each transformation, selecting for Ura+ transformants.
The fur genes of 62 transformants selected solely for chloramphenicol resistance were sequenced (average ∼12/targeted site).
Four transformants selected only for chlorampenicol resistance contained frameshift mutations in fur, had resistance phenotypes equivalent to those of isogenic Δfur strains (160R 190S), and were not studied further.
85 transformants selected with Zeocin were analyzed for SK expression by LNA assay.
Approximately 20 colonies with positive transformants were selected for each sample, and each transformant was cultivated separately in LB broth at 37°C for 12 h.
Transformants were selected for growth on nutrition selection plates (-histidine, -leucine and -tryptophan) containing different concentrations of DHT.
The ligation mixture was used to transform the naturally transformable S. gordonii «Challis» strain V288 and transformants were selected for Cm resistance.
Plasmids were transformed into RJD 4189 and then transformants were selected for growth on 5FOA-containing media.
Briefly, strain E. coli DH5α was co-transformed with plasmids pAT101 and pBD414V and transformants were selected for resistance to both kanamycin (50 μg ml−1) and spectinomycin (100 μg ml−1).
