This is supported by the observation that 10 out of the 12 primary transformants produced with the SP-NaD1ΔCTPP construct had an abnormal phenotype.
All transformants produced significantly higher levels of citric acid.
The transformants produced bright green-fluorescence and biosynthesis was independent of oxygen.
It is unclear why transformants produced with pPsaD-GLuc (PanTJ…) didn't produce significant luciferase activity.
Mycelia from the transformants producing the highest amounts of GFP were harvested by filtration.
The Bcy1HA fusion produced by transformants is functional because the BCY1 HA / BCY1 strain was insensitive to heat shock (2 h at 50°C) (data not shown), unlike a BCY1/ bcy1 strain [ 4].
In batch fermentation the transformant produced up to 3.1 g/L of 2,3-butanediol, as well as acetone, butanol and ethanol (ABE, 17.8 g/L) in amounts similar to those produced by the wild type strain.
The alkanes produced by the transformants and the parent strain were analyzed by GC/MS.
However, the spores produced by SM101::ssp4 transformants expressing the Ssp4D36N mutant had lower resistance against both heat and nitrous acid.
These differences allow discrimination from the wild type GvpC protein (see in Figure 6, Panel A), and are consistent with an expected differentially increased molecular weight of the recombinant GvpC proteins produced by the transformants.
