Sentence examples for transformants out of from inspiring English sources

Five transformants out of 18 lines observed were rated as sterile.

As shown in Figure 4, 96% (45 out of 46) transformants showed significant GFP fluorescence that was regulated by thiamine, indicating the GRC efficiency was dramatically increased in this strain.

In addition, we occasionally found that CPBF1-HA was distributed to the vacuolar membranes without colocalization with Sec61α (Fig. 3B, upper panel, inset) in ∼ 20% of CPBF1-HA transformants (4 out of 23 cells).

A total of 29 independent transformants were examined, out of which 5 lines showed strong growth defects and sterility following treatment with dexamethasone.

Transformation of naturally competent cells of S. gordonii V288 and GP201, scoring and genetic analysis of transformants was carried out as already described [ 21, 22].

Electroporation and selection of transformants were carried out by using MD and G418.

Confirmed transformants were inoculated into nonselective liquid defined medium, with 40 μM uracil, and incubated overnight at 75 °C to allow loop-out of the plasmid.

Confirmed transformants were inoculated into nonselective liquid defined medium, with 40 μM uracil, and incubated overnight at 75°C to allow loop-out of the plasmid DNA.

A Western blot analysis of KmR transformants was carried out using the anti-SPA monoclonal anti-FLAG M2 antibody (Sigma, St Louis, MO).

No hybridization was detected in the transformants, which ruled out the possibility of any bacterial contamination.

Selection of the primary transformants was carried out on MS medium containing 200 μg/ml kanamycin.