Sentence examples for transformants out from inspiring English sources

Five transformants out of 18 lines observed were rated as sterile.

The ability of SGT1 mutants to maintain cell viability was assessed by curing for pRS316-SGT1 by incubating the transformants on drop-out media without leucine, but containing uracil (25 mg/l) and 5-FOA (0.1%).

To test the interaction between two proteins, the corresponding BD and AD constructs were co-transformed into Saccharomyces cerevisiae Y2HGold (Clontech), and the transformants were plated out on synthetic dropout medium without leucine or tryptophan to select for cells in which both BD and AD fusion proteins were co-expressed.

Electroporation and selection of transformants were carried out by using MD and G418.

Moreover, PCR amplification for these 10 transformants was carried out using specific primers designed for the Vir gene of A. tumefaciens, with four strains of A. tumefaciens as positive controls for eliminating the false-positive caused by the contamination of A. tumefaciens.

A Western blot analysis of KmR transformants was carried out using the anti-SPA monoclonal anti-FLAG M2 antibody (Sigma, St Louis, MO).

Transformants were grown out and competent cells were prepared following Inoue's protocol [ 17].

Selection of the primary transformants was carried out on MS medium containing 200 μg/ml kanamycin.

No hybridization was detected in the transformants, which ruled out the possibility of any bacterial contamination.

Forty-six (39.7 %) transformants were filtered out by an obvious fluorescent signal, indicating they were ectopic insertion mutants.

Transformants were struck out immediately (1× streakout) on −leu 5-fluoroorotic acid media to select for loss of the CDC13 -covering plasmid.